rabbit ab to rab7 (d95f2) xp antibody  (Cell Signaling Technology Inc)

Journal: Journal of Virology

Article Title: IFITM proteins are key entry factors for porcine epidemic diarrhea coronavirus

doi: 10.1128/jvi.02028-24

Figure Lengend Snippet: Interaction and colocalization of the PEDV S protein with IFITM proteins. ( A ) Co-IP assays were performed to assess the interaction between the PEDV S1 protein and human or porcine IFITM proteins. HEK293T cells were cotransfected with plasmids encoding PEDV S1-Fc and various HA-tagged IFITM proteins. At 24 h post-transfection, the cells were harvested. Immunoprecipitation was conducted using an Fc tag antibody, and the presence of IFITM proteins was detected by Western blotting (IB: Anti-HA). Whole-cell lysates (WCLs) were analyzed to confirm expression levels (IB: Anti-Fc, Anti-HA, Anti-β-Tubulin). The Co-IP was repeated three times, yielding similar results. ( B ) Confocal microscopy images showing the intracellular localization of porcine IFITM1 in LLC-PK1 cells are presented. IFITM1 (red) colocalizes with clathrin (green), EEA1 (green), Rab7 (green), and LAMP7 (green), as indicated in the merged images. DAPI (blue) was used to stain the nuclei. ( C ) Confocal microscopy images demonstrating the colocalization of the PEDV S protein (red) with HA-IFITM1 (green) in LLC-PK1 cells are shown. The merged image shows the nuclei stained with DAPI (blue).

Article Snippet: Additional antibodies included EEA1 mouse monoclonal antibody (cat#: 68065-1-Ig), HA tag mouse monoclonal antibody (cat#: 66006-2-Ig), Rab7 (D95F2) XP rabbit monoclonal antibody (cat#: 9367s), and CLTC monoclonal antibody (cat#: 66487-1-Ig) from Proteintech.

Techniques: Co-Immunoprecipitation Assay, Transfection, Immunoprecipitation, Western Blot, Expressing, Confocal Microscopy, Staining

Journal: Cells

Article Title: AMPK Activation Downregulates TXNIP, Rab5, and Rab7 Within Minutes, Thereby Inhibiting the Endocytosis-Mediated Entry of Human Pathogenic Viruses.

doi: 10.3390/cells14050334

Figure Lengend Snippet: Figure 7. AMPK activation downregulates Rab5, Rab7, and TXNIP rapidly. Confocal microscopy of Rab5 (magenta), Rab7 (magenta), and TXNIP (magenta) after SA (A,C), AMPK (A), ULK activator (A), and cycloheximide (B) treatment. (C) Analyses of the time-dependent downregulation of Rab5, Rab7, and TXNIP. Scale bars: 20 µm.

Article Snippet: Antibodies: Anti-PVR (CD155) Antibody, clone 4B3, ZooMab® Rabbit Monoclonal recombinant, Cells 2025, 14, 334 4 of 22 Sigma Aldrich; anti-LDL Receptor Antibody, clone 2N19, ZooMab® Rabbit Monoclonal recombinant, Sigma Aldrich (Taufkirchen, Germany); anti-Rab5A Antibody #2143, Cell Signaling; anti-Rab7 (D95F2) XP® Rabbit mAb #9367, Cell Signaling; and anti-TXNIP Antibody, clone 1K14 ZooMAb® Rabbit Monoclonal, Sigma Aldrich; anti-β-Actin Antibody, clone 6L12, ZooMAb® Rabbit Monoclonal, Sigma Aldrich; anti-SARS-CoV-2 Spike Protein (S1-NTD) Antibody #56996, Cell Signaling, anti-SARS-CoV/SARS-CoV-2 NSP8 Monoclonal Antibody (5A10), Invitrogen; and anti-LAMP1 (D4O1S) Mouse mAb #15665, Cell Signaling; anti-GRP78 Polyclonal Antibody, Invitrogen.

Techniques: Activation Assay, Confocal Microscopy

Journal: bioRxiv

Article Title: A Novel TRPC6 Mutation Causes Autosomal Dominant FSGS

doi: 10.1101/2025.02.11.637765

Figure Lengend Snippet: iPS-cell derived podocytes have reduced expression of slit diaphragm proteins, ER congestion, and reduced lysosomes. Panel A shows representative immunoblots of podocyte markers including Nephrin, Synaptopodin, TRPC6, Podocin, CD2AP, GLEPP1, and WT1, the housekeeping protein GAPDH. Panel B shows the quantification of immunoblots for the podocyte markers. Data represents three biologically independent experiments and is defined as ± standard error of the mean. We performed one-way ANOVA with Dunnett’s multiple comparison post hoc analyses with a family-wise alpha threshold of 0.05. Panel C shows electron micrograph of differentiated podocytes with protein aggresomes in the ER of Duke 5 and Duke 7 podocytes. Panel D shows LAMP1 vesicles, Podocin, and TRPC6 expression in podocytes. The LAMP1 vesicle number remains unchanged in the podocytes. Panel E shows lysosomal biogenesis-associated RAB7 expression in the podocytes. RAB7 expression was reduced in the mutants. Data is representative of three biologically independent experiments and the data is defined as ± standard error of the mean. We performed one-way ANOVA with Sidak’s multiple comparison post hoc analyses with a family-wise alpha threshold of 0.05. Scale bar: Panel C, top row 1000 µm, bottom row 500 nm; Panel D, top row 20 µm, bottom row 5 µm; Panel E, 100 µm.

Article Snippet: The primary antibodies used included guinea pig Anti-Nephrin (ARP; #GPN02, 1:500), rabbit Anti-Podocin (Abcam, mAb #ab50339, 1:1000), rabbit Anti-TRPC6 (Abcam, pAb #ab228771) and mouse Anti-CD2AP (B-4) (Santa Cruz, #sc-25272), Calnexin (C5C9) Anti-Rabbit (Cell Signaling, mAb #2679), LAMP1 (D2D11) XP Anti-Rabbit (Cell Signaling, mAb #9091), RAB7 (D95F2) XP and Anti-Rabbit mAb (Cell Signaling, mAb #9367).

Techniques: Derivative Assay, Expressing, Western Blot, Comparison

Journal: Current Biology

Article Title: Systems mapping of bidirectional endosomal transport through the crowded cell

doi: 10.1016/j.cub.2024.08.026

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-Rab7 (D95F2) , Cell signaling Technology , Cat#9367; RRID: AB_1904103.

Techniques: Recombinant, CRISPR, Sequencing, Introduce, Cloning, Software